Translocations involving the immunoglobulin loci are recurring events in various subtypes of B-cell lymphomas.
In addition to translocations involving the IGH locus, variant translocations have been described in 5-10% of B-cell neoplasms involving either the immunoglobulin kappa (IGK) light chain locus at 2p11.2 or the lambda light chain (IGL) at 22q111,2. The best known translocations involving IG light chain loci are the variant Burkitt's translocations t(2;8)(p12;q24) and t(8;22)(q24;q11) present in up to 21% of all Burkitt's lymphomas3. Other translocations involve the BCL6 oncogene: the t(2;3)(p12;q27) and t(3;22)(q27;q11) and BCL2 locus: t(2;18)(p12;q21) and t(18;22)(q21;q11)5.
Translocations involving the IG light chain loci usually lead to breakage within the joining region of the respective locus2. IGK has a largely duplicated structure with duplicate gene regions being 96-100% identical. The IGK proximal copy is present in a 542kb contig with 22 potentially functional variable (IGKV) gene segments and 18 pseudogenes plus five joining (J)- segments and one IGK constant (IGKC) gene segment. The distal copy is a 433kb contig with 21 potentially functional IGKV gene segments and 15 pseudogenes. The two contigs are separated by a DNA sequence of 800kb without any IGKV segments1.
1. Poulseu TS et al., Leukemia 2002;16:2148-55
2. Martin-Subero JI et al., Int J Cancer 2002;98:470-4
3. Kornblau SM et al., Hematol Oncol 1991;9:63-78
4. Chaganti SR et al., Genes Chromosomes Cancer 1998;23:323-7
5. Tashiro S et al., Oncogene 1992;7:573-7
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This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples, or formalin-fixed paraffin-embedded (FFPE) tissues.
*Disease information supported by the literature and is not a reflection of the intended purpose of this product.