MYH11 (16p13.1) breakpoint probe

Proximal Probe: Green Fluorophore
Distal Probe: Red Fluorophore

Inv(16)(p13q22) and t(16;16)(p13;q22), often missed by conventional cytogenetics in a third of patients, are found in 10% of patients with de novo acute myeloid leukaemia (AML) and are most commonly associated with M4 with abnormal eosinophilia. In these rearrangements the core binding factor beta gene on 16q22 is fused to the smooth muscle heavy chain gene (MYH11) on 16p13 giving a transcribed fusion gene. The rearrangements are recognised as positive prognostic factors in de novo adult AML.

The MYH11 Probe mixture contains sequences proximal and distal to the 16p breakpoint which are dual labelled, in green and red respectively.