13q14.3 deletion probe

D13S319-D13S25: Red Fluorophore
13q Subtelomere Specific probe (clone 163C9): Green Fluorophore

Deletions in 13q14 are common genetic alterations in B-cell neoplasm, from Acute Lymphoblastic Leukaemia to Multiple Myeloma and non-Hodgkin's Lymphoma. Deletions are observed in 30% of karyotypically normal Multiple Myeloma patients and in over 20% of B-cell Chronic Lymphocytic Leukaemia (CLL) patients. A further 20% have microscopic deletions and 10% exhibit homozygous loss. The large (~2 Mb) deletions are telomeric to the Retinoblastoma gene, flanked by the markers D13S273 and D13S25. CLL patients with a 13q14.3 deletion have a better prognosis than patients with trisomy 12.

The 13q14.3 deletion probe covers the region 100 Kb centromeric to D13S319 through to the marker D13S25. The probe mix is directly labelled, with 13q14.3 in red and the 13qter control probe in green.