PML/RARA translocation probe

PML (15q22): Red Fluorophore
RARa (17q21): Green Fluorophore

The translocation t(15;17)(q22;q21) is the diagnostic hallmark of Acute Promyelocytic Leukaemia and leads to the formation of PML-RARa and RARa-PML fusion genes. The PML-RARa fusion protein is typically transcribed from the derived chromosome 15, but RARa-PML is not expressed in 25% of patients. Establishing the existence of the t(15;17) or the PML-RARa rearrangement, is fundamental for optimal treatment of patients. Interstitial insertion events have also been detected when chromosomes 15 and 17 have appeared normal by conventional cytogenetics. The PML/RARa Translocation Probe mixture is directly labelled and contains the 5' region of the PML gene including exons 1 and 2 (red) and sequences from exon 2 to 9 from RARa (green). This type of probe may be used to identify the PML/RARa fusion gene.