Satellite probes

Cytocell offers a complete range of Satellite probes available in the Aquarius liquid format. The probes are chromosome specific sequences generated from highly repeated human satellite DNA sequences located in the centromeric, pericentromeric or heterochromatic regions of each chromosome. They are available independently and directly labelled in either a red or green fluorophore (Texas Red or FITC spectra respectively).

The probes are produced in a concentrated form to allow the mixing, if required, of up to 3 other satellite probes in the same hybridisation.
The kits are supplied in an economical 5 test format and come complete with hybridisation solution and DAPI counterstain. The protocol is rapid and simple and follows a co-denaturation of the FISH probe and target DNA simultaneously.

The probes are designed for use in fluorescence in situ hybridisation analysis of human metaphase chromosomes from cultured peripheral blood cells.

Dual Labelled Satellite Probe Sets

We also offer two dual labelled X&Y probe sets, available as Aquarius directly labelled probes in a 10 test format. They may be used to identify X & Y human chromosomes in interphase cells and metaphases chromosomes.

• XYc: DXZ1 (Xp11.1-q11.1) directly labelled with a green fluorophore (FITC spectrum) and DYZ3 (Yp11.1-q11.1) with a red fluorophore (Texas Red spectrum).
• XYq: DXZ1 (Xp11.1-q11.1) directly labelled with a green fluorophore (FITC spectrum) and DYZ1(Yq12) with a red fluorophore (Texas Red spectrum).

Related Products

Chromoprobe Multiprobe-I System

The Chromoprobe Multiprobe-I System allows the simultaneous analysis of all chromosome specific satellite sequences on one slide in one single hybridisation. It combines a Multiprobe glass device of 24 squares with a complete set of alpha and classical satellite probes. Each square of the Multiprobe carries a satellite probe for one of the 24 chromosomes. The probes are directly labelled in a red fluorophore (Texas Red spectrum).