Chromoprobe Multiprobe® - CLL System

The Chromoprobe Multiprobe Leukaemia Panels have been designed to provide as much information about as many chromosomal rearrangements as possible for each of the Leukaemias targeted on slide in a single hybridisation experiment.

  • Eight chromosome rearrangements detected simultaneously in a single FISH

Knowledge of the precise rearrangement involved has been shown to have important implications for the prognosis for the patient and can thus provide vital diagnostic and patient management information. These devices have therefore been designed to help fill the gap between diagnosis and prognosis, helping, therefore to direct the treatment of these important and relatively common diseases.

Chronic lymphocytic leukaemia is the most common of the B-cell lineage lymphoproliferative disorders, accounting for about 30% of all cases in the USA and Europe, though it is extremely rare in the orient. Where the disease is prevalent, it occurs almost exclusively in the middle-aged and elderly and is therefore rarely observed in children. The disease is found in both B- and T-cell lineages, though 95% of cases are of B-cell origin. Until relatively recently, study in to the chromosome rearrangements that may lead to CLL had been hampered by the low spontaneous mitotic rate of these tissues.

The introduction of B-cell mitogens into the field improved the number of cells analysable by conventional cytogenetics and increased the number of rearrangements implicated in the disease. Trisomy 12, deletions of 13q and the 14q marker chromosomes were the first to be detected, though it was not until the introduction FISH in the study of Leukaemia that the true incidence and therefore relevance of these rearrangements began to become clear. Further molecular genetic studies in recent years have increased the numbers of chromosome rearrangements involved in CLL, showing that deletions of 6q (MYB), 17p (p53) and 11q (ATM) are also important.

2,5 or 10 devices Cat No. PMP018/017/016

Click to see our CLL presentation

  • Fish detects rearrangements in 72% of patients. Conventional Cytogenetics successful in 31%
  • Detects Balanced chromosome rearrangements
  • Interphase analysis:no culturing necessary
  • Eight chromosome rearrangements detected simultaneously in a single FISH reaction on a single microscope slide
  • Detection easily automated: signals show as spots
  • Diagnosis provides prognosis
  • Diagnosis directs therapy