Chromoprobe Multiprobe® - T System

Chromosomal rearrangements involving the ends of chromosomes have emerged as an important cause of genetic disease given the gene-rich regions adjacent to the telomeres. The importance of such subtelomeric chromosome rearrangements has been clearly shown by their observed association with unexplained mental retardation and congenital abnormalities and with the establishment of new retardation syndromes involving subtelomeric deletions such as the 1p36 deletion syndrome and the 22q13.3 deletion syndrome.

The Chromoprobe Multiprobe-T System combines Cytocell's Chromoprobe Multiprobe technology with a complete set of subtelomere probes to allow the simultaneous analysis of all subtelomeric regions of every chromosome on one slide in one hybridisation.

The Chromoprobe Multiprobe-T device is divided into 24 square areas. Each square carries subtelomere specific probes for both the p-arm and the q-arm of one of the 24 chromosomes (except for the acrocentric chromosomes). The p-arm and the q-arm probes for each chromosome are labelled in different colours, with two spectrally independent fluorophores (green and red respectively), and are located together in the corresponding Multiprobe square. The probes provide each other with an internal hybridisation control and effective chromosome identification.

Cytocell's subtelomere specific probes have been chosen from the most distal unique sequence to provide the best possible chromosome specificity whilst also being the most subtelomeric probes available that are capable of being used routinely to examine subtelomere enumeration and integrity.

The isolation of these clones, together with the technique of fluorescence in situ hybridisation (FISH) and the Chromoprobe Multiprobe technology, provide a practical cytogenetic solution for routine subtelomere screening.

The Chromoprobe Multiprobe System is designed for fluorescence in situ hybridisation (FISH) of metaphase chromosomes from cultured peripheral blood cells.

Related Products

The subtelomere specific probes applied on the Chromoprobe Multiprobe-T device are also available in the Aquarius liquid format.

Aquarius Single Subtelomere Specific Probe range

The Aquarius Subtelomere Specific probe range consists of subtelomere specific probes for both p-arm and q-arm of each chromosome. The probes are available independently and are directly labelled in either a green or a red fluorophore (FITC or Texas Red spectra respectively).

2,5 or 10 devices Cat No. PMP009/008/007

  • Identify subtle chromosome rearrangements in patients suspected of carrying a chromosome anomaly quickly and easily.
  • Single slide assay with all 24 chromosomes represented, detecting balanced chromosome rearrangements otherwise missed by alternative molecular biology techniques.
  • p and q probes in different colours for the same chromosome allows accurate interpretation of results.
  • Probes are reversibly bound to the glass device, ready to use, saving valuable time and resources.
  • The single slide per patient provides an easy and permanent record of the probe hybridisation results.
  • Results are visible in situ using a conventional fluorescence microscope so there is no need for additional interpretation or expensive equipment.