Aquarius FISH Procedure
The Aquarius® range consists of directly labelled FISH probes for constitutional cytogenetic and oncology applications.
Spot slide with 10μl of cell sample and dehydrate.
Apply 10μl of Aquarius probe onto dehydrated cell sample.
Place coverslip onto slide and seal.
Denature on a hotplate at 75°C (+/-1°C) for 2 minutes. Hybridise the slide in a humid, lightproof container at 37°C (+/-1°C) overnight.
Wash with rapid, formamide-free stringency washes.
Apply DAPI counterstain provided and view under a fluorescent microscope.