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FISH general

How big are Cytocell FISH probes? How is this decision made?

All our probe products are optimised to make them suitable for FISH. We generally cut all our probes up to fragments of between 200 and 2000bp depending on the product. The size of the signal depends on the length of the DNA sequence recognised by the probe when all fragments recognise their target.

What is the coverage of the Whole Chromosome paint probes (LPP/AMP)? Are both euchromatin/heterochromatin regions covered?

Both mouse and human Whole Chromosome Painting probes are specific for each chromosome and cover the entire chromosome other than centromere regions. They are derived from specific flow-sorted chromosomes and have been amplified using degenerate oligonucleotide primers (DOP). DOP primers can potentially bind to many sequences in the genome such as unique sequence, subtelomere, and heterochromatin as it finds the complementary region along the length of the chromosome.

Therefore, mouse whole chromosome paints provide good coverage and can be used in a wide range of applications in mouse as well as on cross species studies. Human whole chromosome paints provide good coverage and can be used in a wide range of clinical and non-clinical applications.