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FISH protocol: Hybridisation

‘We are having problems with weak centromeric control probe signals, what could be the cause?’

This is likely to be caused by low humidity during the hybridisation step. The FITC (green) fluorophore is more sensitive to these changes and can produce loss of or drop out of control probe signals. The optimal humidity levels for efficient probe hybridisation are 95%+, however we would recommend 95-100% humidity to minimise the risk of weak control signals.