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FFPE Pretreatment Procedure

With ease-of-use and convenience in mind, our simple two stage FFPE slide preparation protocol employs the use of ready-to-use reagents that have been optimised to increase the permeabilisation of cell membranes and facilitate penetration of the desired FISH or CISH DNA probe.

Tissue 1

Deparaffinise slides and rehydrate through an ethanol series.

Tissue 2

Place slides into Tissue Pretreatment solution (Reagent 1), preheated to 100°C.

Tissue 3

Rinse slides and apply Enzyme Reagent provided.

Tissue 4

Dehydrate the slides through an ethanol series and continue with the FISH denaturation and hybridisation protocol.