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IGH/BCL2 Translocation, Dual Fusion

Applications
haematology
Catalogue Numbers
LPH 018-S (5 tests)
LPH 018 (10 tests)

Probe Specification

  • BCL2, 18q21.33, Red
  • IGH, 14q32.33, Green

The IGH/BCL2 product consists of probes, labelled in green, covering the Constant, J, D and Variable segments of the IGH gene, and a 585kb probe, labelled in red, covering the BCL2 and KDSR genes.

Probe Information

The t(14;18)(q32;q21) translocation involving the IGH (immunoglobulin heavy locus) gene at 14q32.33 and the BCL2 (BCL2 apoptosis regulator) gene at 18q21.33 is a recognised recurrent abnormality seen in B-cell malignancies.

IGH-BCL2 rearrangements are observed in 70-95% of follicular lymphoma (FL) cases and 20-30% of diffuse large B-cell lymphoma (DLBCL)1. Presence of the t(14;18) translocation in DLBCL is a predictor of outcome and has a poor prognostic effect2. BCL2 translocations have also been implicated in chronic B-cell lymphoproliferative disease (CLPD) and also occur occasionally in chronic lymphocytic leukaemia (CLL)3.

In our hands, Cytocell FISH probes, have proven to be of the highest quality with bright, easy to interpret signals, thus providing confidence in our results. Cytocell’s customer support is outstanding, as their staff are extremely knowledgeable and truly care about their customers and their customers’ needs. Jennie Thurston, Director of Cytogenetics at Carolinas Pathology Group

References

  1. Tomita N., J Clin Exp Hematop 2011;51(1):7-12
  2. Barrans et al., Clin Cancer Res 2003; 9; 2133
  3. Bassegio L et al., Br J Haematol 2012;158(4):489-98
  4. Arsham, MS., Barch, MJ. and Lawce HJ. (eds.) (2017) The AGT Cytogenetics Laboratory Manual. New Jersey: John Wiley & Sons Inc.
  5. Mascarello JT, Hirsch B, Kearney HM, et al. Section E9 of the American College of Medical Genetics technical standards and guidelines: fluorescence in situ hybridization. Genet Med. 2011;13(7):667-675.
  6. Wiktor AE, Dyke DLV, Stupca PJ, Ketterling RP, Thorland EC, Shearer BM, Fink SR, Stockero KJ, Majorowicz JR, Dewald GW. Preclinical validation of fluorescence in situ hybridization assays for clinical practice. Genetics in Medicine. 2006;8(1):16–23.

Microscope Images

IGH BCL2 Translocation Dual Fusion magnified
Area of Interest*
Lymphoma

Disclaimer

This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples.

*Disease information supported by the literature and is not a reflection of the intended purpose of this product.