IGH/cMYC (MYC) Translocation, Dual Fusion
- cMYC, 8q24.21, Red
- IGH, 14q32.33, Green
The IGH/cMYC product consists of probes, labelled in green, covering the Constant and Variable segments of the IGH gene, and cMYC probes, labelled in red. The cMYC probe mix contains a 220kb probe centromeric to the cMYC (MYC) gene and a second probe covering the 186kb region telomeric to the cMYC gene, including the D8S1644 marker.
The cMYC(MYC)/IGH translocation t(8;14), and the variant forms t(2;8)(p13;q24) and t(8;22)(q24;q11), are found in Burkitt's lymphoma and mature B-cell or Burkitt's type Acute Lymphoblastic Leukaemia (ALL)1.The t(8;14) is the most common and is found in approximately 85% of patients with Burkitt's lymphoma2.
While translocation breakpoints on chromosome 14 are clustered to a narrow region 5' to the intron enhancer of the immunoglobulin heavy chain, the breakpoints on chromosome 8 can occur more than 340kb upstream of MYC, with no preferential site3. The translocation brings MYC into close proximity to the IgH enhancer and results in the up-regulation of MYC4. Over expression of the transcription factor stimulates gene amplification resulting in uncontrolled cell proliferation, which usually occurs in the late event of tumour progression5.
The quality of the products we have received from Cytocell have been excellent. The FISH probes they provide to us give intense, strong signals and are a pleasure to count. What has really stood out however has been the level of support and assistance provided by Cytocell’s application specialists. The team worked very closely alongside our own during the adoption of this product and spent many hours with us perfecting the technique, going above and beyond what I would expect during the transition period. Source BioScience absolutely demand high quality products and service to be able to deliver our results with confidence, and that is what we have received from Cytocell. Neil Ryan, Laboratory Operations Manager at Source BioScience,
1. Berger R and Bernheim A, Cancer Genet Cytogenet 1982;7(3):231-442.
2. Hoffman, Ronald (2009). Hematology : basic principles and practice (5th ed. ed.). Philadelphia, PA: Churchill Livingstone/Elsevier. pp. 1304-13053.
3. Joos et al., Human Molecular Genetics 1992;1(8):625-324.
4. Erikson J et al., Proc Natl Acad Sci USA 1983;80(3):820-4
5. Shou et al., PNAS 2000;97(1):228-33
- Area of Interest*
- ALL, Lymphoma
This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples.
*Disease information supported by the literature and is not a reflection of the intended purpose of this product.