IGH/MAF Translocation, Dual Fusion
- MAF, 16q23.1-q23.2, Red
- IGH, 14q32.33, Green
The IGH/MAF product consists of probes, labelled in green, covering the Constant, J, D and Variable segments of the IGH gene, and MAF probes, labelled in red. The MAF probe mix contains two probes centromeric to the MAF gene (145kb and 91kb) covering part of the WWOX gene and includes the D16S3213 and the D16S3029 markers. The other two probes, telomeric to MAF cover 119kb and 125kb regions and include the RH69965 and the D16S3073 markers respectively.
The translocation t(14;16)(q32.3;q23) involving MAF and IGH is present in 25% of myeloma cell lines1, in 5% of primary Multiple Myeloma samples2,3 and results in high levels of MAF expression. Patients harbouring the t(14;16) appear to have a worse outcome3,4. The majority of the breakpoints are dispersed over a region of approximately 500kb, centromeric to the MAF proto-oncogene at 16q23. Another breakpoint, has been observed that is telomeric to MAF1. Translocations with the centromeric breakpoints place MAF under the control of strong 3' IGH enhancers. Recent gene expression profiling of myeloma cell lines revealed that MAF caused transactivation of cyclin D2 (a promoter of cell cycle progression), thus enhancing proliferation of myeloma cells4.Within or near to the MAF/IGH breakpoint region lies the putative tumour suppressor gene, WWOX, which spans the common chromosomal fragile site 16D (FRA16D) at chromosome 16q23.3-24.1 - a region that is a frequent target for both loss of heterozygosity and chromosomal rearrangements in ovarian, breast, hepatocellular and prostate carcinomas as well as other neoplasias5.
The quality of the products we have received from Cytocell have been excellent. The FISH probes they provide to us give intense, strong signals and are a pleasure to count. What has really stood out however has been the level of support and assistance provided by Cytocell’s application specialists. The team worked very closely alongside our own during the adoption of this product and spent many hours with us perfecting the technique, going above and beyond what I would expect during the transition period. Source BioScience absolutely demand high quality products and service to be able to deliver our results with confidence, and that is what we have received from Cytocell. Neil Ryan, Laboratory Operations Manager at Source BioScience
1. Chesi M et al., Blood 1998;91(12):4457-632.
2. Avet-Loiseau H et al., Blood 2002;99(6):2185-913.
3. Fonseca R et al., Blood 2003;101(11):4569-754.
4. Chang H et al., Leukemia 2007;21:1572-45.
5. Nunez MI et al., BMC Cancer 2005;5:64
- Area of Interest*
This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples.
*Disease information supported by the literature and is not a reflection of the intended purpose of this product.