CLL Plus Screening Panel
A selection of haematology probes and an alpha-satellite probe for Chronic Lymphocytic Leukaemia (CLL).
Alpha Satellite 12 Plus for CLL
- D12Z3, 12p11.1-q11.1, Red
The Alpha Satellite 12 Plus Probe is a repeat sequence probe, labelled in red, which recognizes the centromeric repeat sequence D12Z3.
Trisomy of chromosome 12 is a relatively common chromosome abnormality in B- CLL, being present in around 16% of patients, when evaluated by FISH on interphase and metaphase cells1 and is now thought to have strong prognostic significance2.
This product is also available in 5 (LPH 069-S) and 10 (LPH 069) test kit sizes and has been optimised for overnight hybridisation.
13q14.3 Deletion Probe
- 13q14.2-q14.3, Red
- 13qter, 13q34, Green
The 13q14.2-q14.3 probe, labelled in red, covers the D13S319 and D13S25 markers. The 13qter subtelomere specific probe (clone 163C9), labelled in green, allows identification of chromosome 13 and acts as a control probe.
Deletions of 13q14 are a common event in CLL. This region is found to be heterozygously deleted in 30-60% and homozygously deleted in 10-20% of CLL patients3.
P53 (TP53) Deletion Probe
- P53, 17p13.1, Red
- D17Z1, 17p11.1-q11.1, Green
The P53 probe is 161kb, labelled in red, covers the whole P53 (TP53) gene, extending 66kb telomeric to the gene and covering a region centromeric to the gene, to just beyond the marker D17S655. The probe mix also contains a control probe for the 17 centromere (D17Z1) labelled in green.
P53 (TP53) is a tumour suppressor gene that mediates the apoptosis of damaged cells. It has been found that approximately 17% of B-CLL patients have deletions of the P53 gene4. Patients exhibiting this genotype are associated with a poor prognosis5 as they harbour a proliferating population of damaged cells.
ATM Deletion Probe
- ATM, 11q22.3, Red
- D11Z1, 11p11.1-q11.1, Green
The ATM probe is 182kb, labelled in red, and covers the telomeric end of the NPAT gene and the centromeric end of the ATM gene to just beyond the D11S3347 marker. The probe mix also contains a control probe for the 11 centromere (D11Z1) labelled in green.
The ATM gene is an important regulatory candidate in cell damage management. When it is deleted, damaged cells are neither repaired nor apoptosed and are allowed to proliferate. Screening for deletions of ATM is important to allow informed therapy choices for CLL patients, especially as deletions confer poor prognosis5.
MYB Deletion Probe
- MYB, 6q23.3, Red
- D6Z1, 6p11.1-q11.1, Green
The MYB probe is 183kb, labelled in red, covers the entire MYB gene and a region telomeric to the gene, 137kb beyond the marker AFMA074ZG9. The probe mix also contains a control probe for the 6 centromere (D6Z1) labelled in green.
Deletions of chromosome 6q are found in B-CLL, with breakpoints being reported in bands 6q13, q15 or q21. The MYB gene is essential in haematopoietic cell p roliferation and differentiation. It is located in band 6q23.3 and is provided as a marker for 6q deletion as it is distal to reported deletion breakpoints (6q13, 6q15 and 6q21)6.
The quality of the products we have received from Cytocell have been excellent. The FISH probes they provide to us give intense, strong signals and are a pleasure to count. What has really stood out however has been the level of support and assistance provided by Cytocell’s application specialists. The team worked very closely alongside our own during the adoption of this product and spent many hours with us perfecting the technique, going above and beyond what I would expect during the transition period. Source BioScience absolutely demand high quality products and service to be able to deliver our results with confidence, and that is what we have received from Cytocell. Neil Ryan, Laboratory Operations Manager at Source BioScience
1. Döhner et al., N Engl J Med 2000;343(26):1910-1916
2. Chiorazzi. ASH Education Book; 2012: 2012(1):76-87
3. Hammarsund M et al., FEBS Letters 2004;556:75-80
4. Döhner et al., J Mol Med 1999;77:266-81
5. Foá et al., Haematol 2013; 98(5):675-685
6. Brigaudeau and Bilhou-Nabera . del(6q) abnormalities in lymphoid malignancies. Atlas Genet Cytogenet OncolHaematol. December 1998
- Area of Interest*
This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples.
*Disease information supported by the literature and is not a reflection of the intended purpose of this product.