CLL PROFILER Kit
The Cytocell CLL PROFILER Kit is intended to detect deletions of P53, ATM and D13S319, and gains of the chromosome 12 centromere sequence in peripheral blood or bone marrow samples from patients with B-cell Chronic Lymphocytic Leukaemia (B-CLL).
P53(TP53)/ATM Probe Combination
- P53, 17p13.1, Red
- ATM, 11q22.3, Green
The P53 probe is 161kb, labelled in red and covers the whole P53 gene, extends 66kb telomeric to the gene and covers a region centromeric to the gene, to just beyond the marker D17S655. The ATM probe is 182kb, labelled in green, and covers the telomeric end of NPAT gene and the centromeric end of the ATM gene to just beyond the D11S3347 marker
P53 is a tumour suppressor gene that mediates the apoptosis of damaged cells. It has been found that approximately 17% of B-CLL patients have deletions of the P53 gene1. Patients exhibiting this genotype are associated with a poor prognosis2 as they harbour a proliferating population of damaged cells. The ATM gene is an important regulatory candidate in cell damage management. When it is deleted damaged cells are neither repaired nor apoptosed and are allowed to proliferate. The detection of the ATM deletion in CLL is important as it indicates poor prognosis and can define a patient's therapy3. Deletions of ATM and P53 are the most serious rearrangements involved in CLL and detection of deletions of these genes provides very important information as to the therapy choices for such patients especially since deletions of P53 and ATM provide a poor prognosis4,5.
- D13S319, 13q14.2, Red
- 13qter, 13q34, Blue
- D12Z3, 12p11.1-q11.1, Green
The Chromosome 12 Alpha Satellite Probe is a repeat sequence probe, labelled in green, which recognises the centromeric repeat sequence D12Z3. The D13S319 probe, labelled in red, covers a 156kb region including the entire DLEU1 and most of the DLEU2 genes and the D13S319, D13S272 and RH47934 markers. The 13qter subtelomere specific probe, labelled in blue, allows identification of chromosome 13 and acts as a control probe.
Deletions affecting the 13q14 band are the most frequent genetic abnormalities of B-cell Chronic Lymphocytic Leukaemia (B-CLL)6. This region is found deleted heterozygously in 30-60% and homozygously in 10-20% of CLL patients7. Recently though, the survival rate has been shown to be similar8. Two non-coding RNA genes, DLEU1 and DLEU2, and the genetic marker D13S319, span the pathogenic critical region 13q14.39. DLEU1 is considered to be the most likely CLL-associated candidate tumour suppressor gene within the 13q14 region10. Trisomy of chromosome 12 is the most common chromosome abnormality in B-CLL11,12,13,14 and is now thought to have strong prognostic significance15. The molecular consequences of the additional chromosome are not clear, though it has been shown to be more common in the non-dividing cells rather than mitoses which are frequently found to be cytogenetically normal. This was deduced from FISH studies using centromeric probes for chromosome 1211.
I am grateful for the excellent products I receive from Cytocell at a reasonable price, but more importantly the superb customer support. The speed in which I receive answers or suggestions makes my life as a director much easier and allows me to focus on patient care. The quality and consistency of Cytocell’s probes means I can trust the results, and my clients get their results in a timely manner. Dr. Theresa C. Brown, Director, Cytogenetics Laboratory, Hayward Genetics Center, Tulane University School of Medicine
1. Döhner et al., J Mol Med 1999;77:266-81
2. Döhner et al., Blood 1995; 85(6):1580-1589
3. Tsimberidou et al., Cancer 2009; 115:373–380
4. Guarini et al., Haematol. 2012;97(1):47-55
5. Stilgenbauer and Zenz ASH Education Book 2010 1:481-488
6. Juliusson G et al., N Eng J Med 1990;323:720-4
7. Hammarsund M et al., FEBS Letters 2004;556:75-80
8. Van Dyke DL et al., Br J Haematology 2009;148:544-50
9. Liu Y et al., Oncogene 1997;15:2463-73
10. Wolf S et al., Hum Mol Genet 2001;10:1275-85
11. Anastasi J et al., Blood 1992;79(7):1796-801
12. Aoun P et al., Leuk Lymphoma 2004;45(8):1595-603
13. Bienz N et al., Br J Haematol 1993;85(4):819-22
14. Escudier SM et al., Blood 1993;81(10):2702-7
15. Chiorazzi. ASH Education Book; 2012: 2012(1):76-87
- Area of Interest*
This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples.
*Disease information supported by the literature and is not a reflection of the intended purpose of this product.