Aquarius TeloMark Kit
Subtelomere FISH analysis is a commonly used as an adjunct to routine cytogenetic testing in order to detect small rearrangements involving the telomeric regions of chromosomes. Patients can be referred for telomere analyses for a number of different reasons, including genetic disease, autistic disorders, unexplained mental retardation/developmental delay, recurrent miscarriages and haematological malignancies1-4.
Cytocell's TeloMark kit consists of 41 subtelomere specific probes, three centromere and six locus specific probes (50 different probes in total). The subtelomere probes represent all chromosome ends apart from the p-arms of the five acrocentric chromosomes and with no distinction between the X and Y due to clones being located in the pseudoautosomal regions. All of these probes map to unique regions of the chromosomes, within 850kb of the true telomere.
The probes in TeloMark are provided as 15 separate mixes, either provided individually or as a kit containing all 15. All probes are directly labelled in orange, green, yellow (orange and green combined) or blue.
I first came across Cytocell FISH probes in a previous lab I worked in and I was struck by the quality of the products. Since this time, I have been recommending and introducing Cytocell probes across all application areas — now they are the primary FISH probes used in our lab. They have an excellent range of products and their ready-to-use reagent format saves considerable time. As a matter of fact, at a recent conference there was a discussion about the lack of commercial probes for a particular disorder and I was happy to point the participants in the direction of the Cytocell catalogue, which contains the exact probes required. Elizabeth Benner, Medical Technologist at the University of Arizona Health Network
1. de Vries et al., J Med Genet 2001;38:145-150
2. Knight et al., Am. J. Hum. Genet. 2000; 67:320-332
3. Knight et al., Eur. J Hum. Genet. 1997 Jan-Feb;5(1):1-8
4. Ravan et al., J Med Genet. 2006;43:478-489
This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed peripheral blood samples.