- FIP1L1, 4q12, Green
- CHIC2, 4q12, Red
- PDGFRA, 4q12, Green
The FIP1L1/CHIC2/PDGFRA product consists of a 177kb probe, labelled in green, located centromeric to the FIP1L1 gene, including the D4S1036 marker, a 174kb probe, labelled in red covering the CHIC2 gene and a 174kb probe, labelled in green, located telomeric to the PDGFRA gene, including the D4S956 marker.
Deletion of CHIC2 in patients with idiopathic hypereosinophilic syndrome (HES) and chronic eosinophilic leukaemia (CEL) results in the fusion of FIP1L1 (FIP1-like 1) with PDGFRA (platelet derived growth factor receptor alpha) producing a tyrosine kinase which transforms haematopoietic cells.
In such patients, this activity is inhibited by imatinib mesylate (Gleevec), which is a tyrosine kinase inhibitor.
The diagnosis of the fusion gene can therefore lead to therapeutic choices for the patient1,2.
I first came across Cytocell FISH probes in a previous lab I worked in and I was struck by the quality of the products. Since this time, I have been recommending and introducing Cytocell probes across all application areas — now they are the primary FISH probes used in our lab. They have an excellent range of products and their ready-to-use reagent format saves considerable time. As a matter of fact, at a recent conference there was a discussion about the lack of commercial probes for a particular disorder and I was happy to point the participants in the direction of the Cytocell catalogue, which contains the exact probes required. Elizabeth Benner, Medical Technologist at the University of Arizona Health Network
1. Cools J et al., N Eng J Med 2003;348:1201-14
2. Griffin JH et al., PNAS 2003;100:7830-5
- Area of Interest*
This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples.
*Disease information supported by the literature and is not a reflection of the intended purpose of this product.