IGH/BCL2 Plus Translocation, Dual Fusion
- BCL2, 18q21.33, Red
- IGH, 14q32.33, Green
The IGH/BCL2 Plus product consists of probes, labelled in green, proximal to the Constant, and within the Variable segment of the IGH region and a 585kb probe, labelled in red, covering the BCL2 and KDSR genes.
The t(14;18)(q32;q21) translocation involving the IGH (immunoglobulin heavy locus) gene at 14q32 and the BCL2 (BCL2 apoptosis regulator) gene at 18q21.33 is a recognised recurrent abnormality seen in B-cell malignancies.
IGH-BCL2 rearrangements are observed in 70-95% of follicular lymphoma (FL) cases and 20-30% of diffuse large B-cell lymphoma (DLBCL)1. Presence of the t(14;18) translocation in DLBCL is a predictor of outcome and has a poor prognostic effect2. BCL2 translocations have also been implicated in chronic B-cell lymphoproliferative disease (CLPD) and also occur occasionally in chronic lymphocytic leukaemia (CLL)3.
Not only do Cytocell offer an extensive range of high-quality FISH probes, the customer support is also excellent — providing fast access to all the probes I need. The probes are highly consistent with bright signals allowing easy scoring of results. Dr Eric Crawford, Senior Director, Genetics Associates Inc.
- Tomita N., J Clin Exp Hematop 2011;51(1):7-12
- Barrans et al., Clin Cancer Res 2003; 9; 2133
- Bassegio L et al., Br J Haematol 2012;158(4):489-98
- Arsham, MS., Barch, MJ. and Lawce HJ. (eds.) (2017) The AGT Cytogenetics Laboratory Manual. New Jersey: John Wiley & Sons Inc.
- Mascarello JT, Hirsch B, Kearney HM, et al. Section E9 of the American College of Medical Genetics technical standards and guidelines: fluorescence in situ hybridization. Genet Med. 2011;13(7):667-675.
- Wiktor AE, Dyke DLV, Stupca PJ, Ketterling RP, Thorland EC, Shearer BM, Fink SR, Stockero KJ, Majorowicz JR, Dewald GW. Preclinical validation of fluorescence in situ hybridization assays for clinical practice. Genetics in Medicine. 2006;8(1):16–23.
- Area of Interest*
- CLL, Lymphoma
This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples.
*Disease information supported by the literature and is not a reflection of the intended purpose of this product.