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IGH/CCND1 Plus Translocation, Dual Fusion

Applications
haematology
Catalogue Numbers
LPH 072-S (5 tests)
LPH 072 (10 tests)

Probe Specification

  • CCND1, 11q13.3, Red
  • IGH, 14q32.33, Green

The IGH/CCND1 Plus product consists of probes, labelled in green, proximal to the Constant, and within the Variable segment of the IGH region and CCND1 probes, labelled in red. The CCND1 probe mix contains a 155kb probe centromeric to CCND1 gene, covering a region between the D11S2663 and the D11S4095 markers, and a second probe (162kb) covering the telomeric end of CCND1 gene and the region up to FGF3 gene.

Probe Information

The t(11;14)(q13;q32) translocation involving CCND1 (cyclin D1) gene at 11q13.3 and the IGH (immunoglobulin heavy locus) gene at 14q32.33 is associated with mantle cell lymphoma.

The t(11;14)(q13;q32) rearrangement involving CCND1 and IGH is considered the hallmark of mantle cell lymphoma (MCL)1, the presence of which can be used to aid in the differential diagnosis of CD5+ B-cell lymphoproliferative disorders2.

I first came across Cytocell FISH probes in a previous lab I worked in and I was struck by the quality of the products. Since this time, I have been recommending and introducing Cytocell probes across all application areas — now they are the primary FISH probes used in our lab. They have an excellent range of products and their ready-to-use reagent format saves considerable time. As a matter of fact, at a recent conference there was a discussion about the lack of commercial probes for a particular disorder and I was happy to point the participants in the direction of the Cytocell catalogue, which contains the exact probes required. Elizabeth Benner, Medical Technologist at the University of Arizona Health Network 

References

  1. Vose JM. Am J Hematol. 2013;88(12):1082-8
  2. Ho AK, et al., Am J Clin Pathol 2009;131:27-32
  3. Arsham, MS., Barch, MJ. and Lawce HJ. (eds.) (2017) The AGT Cytogenetics Laboratory Manual. New Jersey: John Wiley & Sons Inc.
  4. Mascarello JT, Hirsch B, Kearney HM, et al. Section E9 of the American College of Medical Genetics technical standards and guidelines: fluorescence in situ hybridization. Genet Med. 2011;13(7):667-675.
  5. Wiktor AE, Dyke DLV, Stupca PJ, Ketterling RP, Thorland EC, Shearer BM, Fink SR, Stockero KJ, Majorowicz JR, Dewald GW. Preclinical validation of fluorescence in situ hybridization assays for clinical practice. Genetics in Medicine. 2006;8(1):16–23.

Microscope Images

IGH CCND1 Plus Translocation Dual Fusion magnified
Area of Interest*
CLL, Lymphoma

Disclaimer

This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples.

*Disease information supported by the literature and is not a reflection of the intended purpose of this product.