MLL/MLLT3 Translocation, Dual Fusion
Translocations involving chromosome 11q23 frequently occur in both acute myeloid leukaemia (AML) and acute lymphoblastic leukaemia (ALL). In most cases, the MLL gene is involved but translocation partners are extremely variable, with 79 direct translocation partners having been molecularly characterised so far.
The most common MLL rearrangement in both AML and ALL involves fusion of the MLL and AFF1 (AF4) genes via a t(4;11)(q21;q23) translocation. Three of the other more common translocations involve the MLLT3 (AF9) gene on chromosome 9, the MLLT1 (ENL) gene on chromosome 19 or the MLLT4 (AF6) gene on chromosome 6.
• MLL/MLLT3(AF9) - t(9;11)(p22;q23)
• MLL/MLLT1(ENL) - t(11;19)(q23;p13.3)
• MLL/MLLT4(AF6) - t(6;11)(q27;q23)
We now have a range of Research Use Only (RUO) Translocation, Dual Fusion probes to allow specific detection of these MLL rearrangements.
I first came across Cytocell FISH probes in a previous lab I worked in and I was struck by the quality of the products. Since this time, I have been recommending and introducing Cytocell probes across all application areas — now they are the primary FISH probes used in our lab. They have an excellent range of products and their ready-to-use reagent format saves considerable time. As a matter of fact, at a recent conference there was a discussion about the lack of commercial probes for a particular disorder and I was happy to point the participants in the direction of the Cytocell catalogue, which contains the exact probes required. Elizabeth Benner, Medical Technologist at the University of Arizona Health Network
• Meyer et al., Leukemia 2013;27(11):2165-76
- Area of Interest*
- ALL, AML
For research use only, not for use in diagnostic procedures.
*Disease information supported by the literature and is not a reflection of the intended purpose of this product.