Deletions of chromosome 6q are found in B-CLL, with breakpoints being reported in bands 6q13, q15 and q21.
The MYB gene (Avian Myeloblastosis Viral Oncogene Homolog) is a homologue of the avian v-myb oncogene and has been shown to be highly expressed in all immature lymphoid and myeloid T-cells, but becomes down regulated in mature T or B-cells1.
The MYB gene is located in band 6q23.3 and is provided as a marker for 6q deletion as it is distal to reported deletion breakpoints (6q13, 6q15 and 6q21).
I first came across Cytocell FISH probes in a previous lab I worked in and I was struck by the quality of the products. Since this time, I have been recommending and introducing Cytocell probes across all application areas — now they are the primary FISH probes used in our lab. They have an excellent range of products and their ready-to-use reagent format saves considerable time. As a matter of fact, at a recent conference there was a discussion about the lack of commercial probes for a particular disorder and I was happy to point the participants in the direction of the Cytocell catalogue, which contains the exact probes required. Elizabeth Benner, Medical Technologist at the University of Arizona Health Network
1. Oh and Reddy. Oncogene. 1999 May 13;18 (19):3017-33
- Area of Interest*
- ALL, CLL, Lymphoma
This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples.
*Disease information supported by the literature and is not a reflection of the intended purpose of this product.