RARα (RARA) Breakapart
- RARα, 17q21.1-q21.2, Red
- RARα, 17q21.1-q21.2, Green
The RARα Breakapart probe mix consists of a 167kb probe centromeric to the RARα gene, including the CASC3 gene, and a 164kb probe, including the telomeric end of the RARα gene as well as the TOP2A and IGFBP4 genes.
Acute Promyelocytic Leukaemia (APL) is associated with chromosomal rearrangements involving the retinoic acid receptor α (RARA) gene, on chromosome 17q21.1-q21.2, and various partner genes situated on different chromosomes2.
In the vast majority of APL cases, the RARA gene fuses with the Promyelocytic Leukaemia (PML) gene located on chromosome 15q24, as a result of a t(15;17) translocation, but RARA fusions with Promyelocytic Leukemia Zinc Finger (PLZF (now known as ZBTB16), 11q23.2), Nucleophosmin (NPM1, 5q35.1), Nuclear Mitotic Apparatus protein 1 (NUMA1, 11q13.4), Myeloid/lymphoid or Mixed Lineage Leukaemia (MLL (KMT2A), 11q23.3) and FIP1-like-1 (FIP1L1, 4q12) genes have also been described1,2,3. The RARAα (RARA) Breakapart probe will detect rearrangements of the RARA gene, irrespective of partner genes or chromosomes involved.
Not only do Cytocell offer an extensive range of high-quality FISH probes, the customer support is also excellent — providing fast access to all the probes I need. The probes are highly consistent with bright signals allowing easy scoring of results. Dr Eric Crawford, Senior Director, Genetics Associates Inc.
1. Melnick A, Licht JD, Blood 1999;93(10):3167-215
3. Rohr et al., Med Oncol 2011;29(4):2345-7
- Area of Interest*
- AML, APL
This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples.
*Disease information supported by the literature and is not a reflection of the intended purpose of this product.