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BCL6 Breakapart

Applications
haematopathology
Catalogue Numbers
LPS 029-S (5 tests)
LPS 029 (10 tests)

Probe Specification

  • BCL6, 3q27.3-q28, Red
  • BCL6, 3q27.3-q28, Green

The BCL6 product consists of a 461kb probe, labelled in red, telomeric to the BCL6 gene, and two green probes, 366kb and 165kb, centromeric to the BCL6 gene.

Probe Information

Rearrangements of the BCL6 gene have been observed in 28.6-35.5% of Diffuse Large B-Cell Lymphoma (DLBCL) and in 6.4-14.3% of Follicular Lymphoma (FL)1.

This translocation results in the dysregulation of BCL6 and may involve the IGH, IGK and IGL genes, although other non-Ig partner genes have also been identified2,3. BCL6 encodes a 95kD nuclear phosphoprotein belonging to the pox virus zinc finger (POZ)/zinc finger (ZF) family of transcription factors4. BCL6 functions as a transcriptional repressor and plays an important regulatory role in lymphoid cell development and function. In the B-cell lineage, the BCL6 protein is expressed only in germinal centre B cells5. Translocations involving band 3q27.3 affect a 4kb major breakpoint region of BCL6 in the first non-coding exon and 5' region of the first intron, and are common in diffuse large B-cell lymphomas. Recently, an alternative breakpoint cluster region located between 245 and 285kb 5' of BCL6 was identified in Non-Hodgkin Lymphoma (NHL) and may be preferentially associated with follicular lymphoma6.

I am grateful for the excellent products I receive from Cytocell at a reasonable price, but more importantly the superb customer support.  The speed in which I receive answers or suggestions makes my life as a director much easier and allows me to focus on patient care.  The quality and consistency of Cytocell’s probes means I can trust the results, and my clients get their results in a timely manner. Dr. Theresa C. Brown, Director, Cytogenetics Laboratory, Hayward Genetics Center, Tulane University School of Medicine

References

1. Ohno H et al., Leuk Lymphoma 1997;27: 53-63

2. Ueda C et al., Leuk Lymphoma 2002;43: 1375-81

3. Akasaka H et al., Cancer Res 2000; 60: 2335-41

4. Chang CC et al., PNAS 1996;93: 6947-52

5. Cattoretti G et al., Blood 1995;85:45-53

6. Bosga-Bouwer AG et al., Genes Chrom Cancer 2005;44: 301-304

Microscope Images

BCL6 Breakapart magnified
Area of Interest*
Lymphoma

Disclaimer

This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples, or formalin-fixed paraffin-embedded (FFPE) tissues.

*Disease information supported by the literature and is not a reflection of the intended purpose of this product.