IGH/BCL2 Translocation, Dual Fusion
- BCL2, 18q21.33-q22.1, Red
- IGH, 14q32.33, Green
The IGH/BCL2 product consists of probes, labelled in green, covering the Constant, J, D and Variable segments of the IGH gene, and four probes labelled in red, positioned centromeric and telomeric to the BCL2 gene.
The IGH/BCL2 fusion gene is brought about by a t(14;18)(q32;q21) translocation and is found in over 88% of follicular lymphoma cases1,2.
The translocation is thought to be brought about by an error in the joining function of the IGH gene, mediated by the recent observation that both IGH and BCL2 are arranged next to each other in 3D space in the normal B lymphocytes2. The translocation breakpoint at the end of the Joining (J) segment, and the subsequent fusion of the BCL2 gene to this region, results in the BCL2 gene coming under the regulatory control of those processes normally involved in maintenance of IGH gene activity3.
The BCL2 gene itself has been shown to be involved in the regulation of apoptosis4.
I first came across Cytocell FISH probes in a previous lab I worked in and I was struck by the quality of the products. Since this time, I have been recommending and introducing Cytocell probes across all application areas — now they are the primary FISH probes used in our lab. They have an excellent range of products and their ready-to-use reagent format saves considerable time. As a matter of fact, at a recent conference there was a discussion about the lack of commercial probes for a particular disorder and I was happy to point the participants in the direction of the Cytocell catalogue, which contains the exact probes required. Elizabeth Benner, Medical Technologist at the University of Arizona Health Network
1. Bernicot et al., Cytogenet Genome Res. 2007;118(2-4):345-52
2. Roix et al., Nature Genetics 2003;34(3):287-91
3. Stoos-Veić et al., Coll Antropol. 2010 Jun;34(2):425-9
4. Sharpe et al., Biochim Biophys Acta. 20041644(2-3):107-13
- Area of Interest*
This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples, or formalin-fixed paraffin-embedded (FFPE) tissues.
*Disease information supported by the literature and is not a reflection of the intended purpose of this product.