N-MYC (MYCN) Amplification
- N-MYC, 2p24.3, Red
- LAF4, 2q11.2, Green
The N-MYC Amplification probe consists of a red 140kb probe spanning the N-MYC (MYCN) gene region at 2p24.3. The LAF4 gene probe in green at 2q11 acts as a control for chromosome 2.
The proto-oncogene N-MYC (MYCN), located on chromosome 2 at 2p24.3, is a transcription factor that plays a role in regulation of cell growth and proliferation.
It is mainly expressed in the developing nervous system and is critical during neural crest embryogenesis. Myelocytomatosis viral homologue (MYCN) becomes rapidly down-regulated as tissues become terminally differentiated and growth-arrested1. Over-expression of MYCN seems to block differentiation and increase cell proliferation. MYCN amplification is the most important unfavourable prognostic factor for neuroblastoma and is amplified in 25% of cases2. Neuroblastoma is a dominating group of neuron tumours and is one of the most common solid tumours in children3.
It was very important for us to have more consistent results with our probes — easy-to-read bright signals and a range of vial sizes, which is much more cost-effective. It also was critical to upgrade our pretreatment kit to expedite the analysis of FFPE samples. We can now complete the process in about 90 minutes. Janet M. Cowan, PhD, Director of the Cytogenetics Laboratory at Tufts Medical Center
1. Thomas WD et al., Int J of Bioch and Cell Biol 2004;36(5):771-5
2. Look et al., J Clin Oncol. 1991 Apr;9(4):581-91
3. Heim and Mitelman, Cancer cytogenetics 2nd Ed. 1995
- Area of Interest*
- Medulloblastoma, Neuroblastoma, Retinoblastoma
This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues.
*Disease information supported by the literature and is not a reflection of the intended purpose of this product.