FFPE Pretreatment Procedure
With ease-of-use and convenience in mind, our simple two stage FFPE slide preparation protocol employs the use of ready-to-use reagents that have been optimised to increase the permeabilisation of cell membranes and facilitate penetration of the desired FISH or CISH DNA probe.
Deparaffinise slides and rehydrate through an ethanol series.
Place slides into Tissue Pretreatment solution (Reagent 1), preheated to 100°C.
Rinse slides and apply Enzyme Reagent provided.
Dehydrate the slides through an ethanol series and continue with the FISH denaturation and hybridisation protocol.