FISH 'n' Tips
We’ve gathered information from our Cytocell experts to share the latest and most helpful tips for fluorescence in situ hybridisation (FISH). Visit this page for timely, proven tips from the FISH experts. Choose from the sections listed below to begin viewing the related tips and tricks.
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Gothami Fonseka, FISH Field Application Support, Europe
Perform tissue enzyme digestion at 37°C on a hotplate for best results.
1. Reagent Storage and Disposal
Tips include advice on storing enzyme solution correctly, how to avoid excessive light exposure, and why you should keep light exposure of probes to a minimum.
2. Sample Preparation
Tips include how to optimise tissue enzyme digestion results, why you should avoid baking or aging of slides, and advice on improving cell morphology.
3. Probe Application
Tips include how to avoid creating a patchy hybridisation, why its a good idea to clean your slides in 70% ethanol before use, and why long pipette tips can help prevent cross contamination.
Tips include why you should use calibrated temperature-measuring devices and why you should test ramp up and ramp down temperature times periodically.
Tips include how to prevent your chromosomes appearing distorted, how to maintain optimimal results from hybridisation units, and advice if you are using an incubator for hybridisation.
6. Post Hybridisation Washes
Tips include advice on measuring the temperature of washing solutions and why you should rinse your slides in a dehydration series of EtOH washes.
7. Probe Analysis
Tips include why you should just use one type of immersion oil in your laboratory and advice on maintenance for your fluorescent microscope filter.
8. General Tips
Tips include the benefit of using dedicated solution jars and how to produce sharper, more distinct probe signals by following one simple step.