FISH 'n' Tips: Denaturation
View our collection of FISH 'n' Tips for the denaturation stage of the fluorescence in situ hybridisation (FISH) protocol. Tips include why you should use calibrated temperature-measuring devices and why you should test ramp up and ramp down temperature times periodically.
Faidra Partheniou, Medical Affairs Manager
Use calibrated temperature-measuring devices to regularly QC hybridisation units; there may be significant slot to slot variability; this can get worse over time leading to poor results.
Ashley Hart, FISH Field Application Scientist, US & Canada
Test ramp up and ramp down temperature times periodically as part of equipment QC on automated hybridisation units, to ensure they are within manufacturer specifications.