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FISH 'n' Tips: Denaturation

Section 4 Denaturation v2

View our collection of FISH 'n' Tips for the denaturation stage of the fluorescence in situ hybridisation (FISH) protocol. Tips include why you should use calibrated temperature-measuring devices and why you should test ramp up and ramp down temperature times periodically. 

Faidra Partheniou, Medical Affairs Manager

Faidra

Use calibrated temperature-measuring devices to regularly QC hybridisation units; there may be significant slot to slot variability; this can get worse over time leading to poor results.

Ashley Hart, FISH Field Application Scientist, US & Canada

Ashley Hart

Test ramp up and ramp down temperature times periodically as part of equipment QC on automated hybridisation units, to ensure they are within manufacturer specifications.