FISH 'n' Tips: General Tips
View our collection of general FISH 'n' Tips for the fluorescence in situ hybridisation (FISH) protocol. Tips include the benefit of using dedicated solution jars and how to produce sharper, more distinct probe signals by following one simple step.
Steve Chatters, Acting Head, Regulatory and Medical Afairs
Periodically washing solution jars and ensuring adequate detergent removal can help reduce background issues.
Jennifer Golding, Global Product Manager - FISH
Having dedicated solution jars (e.g. Coplin jars only for hot wash) for processing steps can prevent poor results due to contamination with incompatible solutions.
Ashley Hart, FISH Field Application Scientist, US & Canada
Have you tried placing counterstained FFPE slides in a -20°C freezer for minimum of 20mins after FISHing? Some labs say this helps to produce sharper, more distinct probe signals.