FISH 'n' Tips: Hybridisation
View our collection of FISH 'n' Tips for the hybridisation stage of the fluorescence in situ hybridisation (FISH) protocol. Tips include how to prevent your chromosomes appearing distorted, how to maintain optimimal results from hybridisation units, and advice if you are using an incubator for hybridisation.
Daniela Johnanes, FISH Field Application Support, Europe
Test ramp up and ramp down temperature times periodically as part of equipment QC on automated hybridisation units, to ensure they are within manufacturer specifications.
Steve Chatters, Acting Head, Regulatory and Medical Afairs
Maintaining humidity strips on automated hybridisation units according to manufacturer recommendations can help ensure optimal results from these units.
Jennifer Golding, Global Product Manager - FISH
Automated hybridisation units may fail to provide sufficient humidity, leading to poor hybridisation. Try using humidified chambers, which can attain consistently-higher humidity levels.
Gothami Fonseka, FISH Field Application Support, Europe
If your chromosomes appear distorted this may be because the coverslip was not sealed. By sealing the coverslips prior to hybridisation, this improves the overall chromosome morphology.
Faidra Partheniou, Medical Affairs Manager
If you are using an incubator for hybridisation utilise a darkened, humidified chamber which has been pre-moistened (wet tissue) and pre-warmed.