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FISH 'n' Tips: Probe Analysis

Section 7 Analysis

View our collection of FISH 'n' Tips for the probe analysis stage of the fluorescence in situ hybridisation (FISH) protocol. Tips include why you should just use one type of immersion oil in your laboratory and advice on maintenance for your fluorescent microscope filter.

Ashley Hart, FISH Field Application Scientist, US & Canada

Ashley Hart

Many types of fluorescent microscope filter degrade over time and should be treated as a consumable. Ensure regular maintenance and replace where necessary. 'Sputter' type filters are now available that last a lifetime.

Faidra Partheniou, Medical Affairs Manager

Faidra

Liquid light-guides in modern fluorescent light sources will degrade over time, resulting in reduced light transmission, and should be treated as a consumable. Make sure there are no sharp bends in the light-guide, schedule regular maintenance and replace when necessary.

Gothami Fonseka, FISH Field Application Support, Europe

Gothami

Try to use just one type of immersion oil in your laboratory; different types of oil may be immiscible. Immersion oil of one type remaining on a microscope objective may result in a ‘milky’ appearance and a reduction of light transmission when it comes in contact with a slide with a different type of immersion oil.