FISH 'n' Tips: Reagent Storage and Disposal
View our collection of FISH 'n' Tips for the reagent and storage stage of the fluorescence in situ hybridisation (FISH) protocol. Tips include advice on storing enzyme solution correctly, how to avoid excessive light exposure, and why you should keep light exposure of probes to a minimum.
Daniela Johnanes, FISH Field Application Support, Europe
When not in use, ensure that the enzyme solution is stored correctly at 2-8°C to preserve the enzyme activity.
Ashley Hart, FISH Field Application Scientist, US & Canada
Keep light exposure of probes to a minimum as this can cause photodegradation.
Steve Chatters, Acting Head, Regulatory and Medical Afairs
Use a working reagent box that you take from the freezer instead of all of the probes to minimise light exposure/freeze thaws. Place the box back in the freezer as soon as you remove the probes you need for the day.
Gothami Fonseka, FISH Field Application Support, Europe
Aliquot probe in to smaller vials to avoid excessive light exposure. Only take the probe to be used from the freezer.