Multiprobe FISH Procedure
The Chromoprobe Multiprobe® System is an extension of Cytocell's proprietary Chromoprobe® technology, whereby DNA FISH probes are reversibly bound to the surface of a glass device. This approach not only simplifies the whole FISH procedure but also renders it safer and quicker to use.
Soak the slides in 100% methanol, then polish dry with a lint free cloth.
Spot 2μl (or 4μl for an 8 square device) of cell sample onto alternate squares of the supplied slide.
Once dry, fill in the remaining squares with the cell sample and check using phase contrast.
Place slides in 2xSSC for 2 minutes and then dehydrate through an ethanol series.
Spot 1μl (or 2μl for an 8 square device) of supplied hybridisation solution onto each square of the device.
Carefully lower spotted slide onto the device.
Check the temperature of the hotplate using the slide surface thermometer provided. Denature the slide/device at 75°C for 2 minutes (or 5 minutes for OctoChrome™ device)
Place slide/device in hybridisation chamber supplied and float on the surface of a clean 37°C waterbath overnight.
Wash in 0.4xSSC at 72°C for 2 minutes, then 2xSSC/0.05% Tween at room temperature for 30 seconds.
Apply DAPI counterstain provided and view under a fluorescence microscope.