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Pathology FFPE FISH Probe Protocol

Watch our brilliant Field Application Specialist, Gothami Fonseka, demonstrate the fluorescence in situ hybridisation (FISH) protocol for OGT's Cytocell® pathology FISH probes, including the tissue pretreatment stage.

Either watch the protocol in full or choose a specific step of the protocol.

Full Pathology FFPE FISH Probe Protocol

Watch the full pathology FFPE FISH probe protocol from start to finish.

https://youtu.be/2_c6tJ56pXU

Step 1. De-paraffinisation

Watch our video for the de-paraffinisation step of the pathology FFPE FISH probe protocol.

https://youtu.be/NI6I6laLLIY

Step 2. Re-hydration

Watch our video for the re-hydration step of the pathology FFPE FISH probe protocol.

https://youtu.be/kowvq2pEc-M

Step 3. Heat Pretreatment

Watch our video for the heat pretreatment step of the pathology FFPE FISH probe protocol.

https://youtu.be/FM6RnkEFtFA

Step 4. Enzyme Digestion

Watch our video for the enzyme digestion step of the pathology FFPE FISH probe protocol.

https://youtu.be/E2wL4tD5n-k

Step 5. Probe Application

Watch our video for the probe application step of the pathology FFPE FISH probe protocol.

https://youtu.be/zWoINe50eaU

Step 6. Denaturation and Hybridisation

Watch our video for the denaturation and hybridisation step of the pathology FFPE FISH probe protocol.

https://youtu.be/6ipZlX1PfSY

Step 7. Post Hybridisation Washes

Watch our video for the post hybridisation wash step of the pathology FFPE FISH probe protocol.

https://youtu.be/Qeb3lno5ODI

Step 8. Counterstain and Analysis

Watch our video for the counterstain and analysis step of the pathology FFPE FISH probe protocol.

https://youtu.be/_8AJn1HDwDs

Disclaimer

This document and its content are © Oxford Gene Technology IP Limited - 2020. All rights reserved. Trademarks: OGT™ (Oxford Gene Technology IP Limited); Cytocell® (Cytocell Ltd).

Always refer to the instructions for use supplied with the product. Further validation is required for use in a diagnostic setting.

The information in this protocol is based on independent validation carried out by Cytocell on a representative set of tissues using the Cytocell Tissue Pretreatment Kit (LPS100) and should be used for guidance purposes only.

The Cytocell Tissue Pretreatment Kit (LPS 100) may not be available in your region.