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What is FISH?

The study of chromosomes in the detection of genetic abnormalities is called cytogenetics. Fluorescence in situ hybridisation (FISH) combines conventional cytogenetics with molecular genetics and can be used in haematology, pathology and in constitutional cytogenetics.

FISH is a technique that uses fluorescently labelled DNA sequences (probes) to assess specific regions of interest, such as genes and centromeres using fluorescent microscopy.

The DNA sequence of the FISH probe is complementary to the region of interest. When using a FISH probe, if the region of interest is present, the probe will bind (hybridise) to the target DNA sequence, which can then be visualised using fluorescent microscopy – indicating the presence of the gene/centromere etc. If the region of interest has been deleted, the probe will not bind to the target DNA and so will not be visualised. A breakapart or a translocation FISH probe can be used to detect whether a gene has been rearranged.

What is FISH ROW