MLL/AFF1 Translocation, Dual Fusion
The t(4;11)(q21;q23) translocation that involves the MLL (KMT2A) gene, on chromosome 11q23.3, and the AFF1 gene, on chromosome 4q21.3-q22.1, is the most frequently observed translocation involving the MLL gene in Acute Lymphoblastic Leukaemia (ALL)1.
The t(4:11) translocation results in the generation of two reciprocal fusion genes: MLL/AFF1 and AFF1/MLL – the leukaemic properties of the first have been documented but the role of the AFF1/MLL fusion protein is still under debate2,3,4.
The 2011 Acute Lymphoblastic Leukaemia Best Practice Guidelines5 state that: “The t(4;11)(q21;q23) is associated with a poor prognosis, and patients with this translocation may be treated on the high risk arm of MRC protocols”…“If chromosome analysis is unsuccessful but FISH indicates a rearrangement of MLL, then further attempts to identify the t(4;11) must be made.”
The MLL/AFF1 Translocation, Dual Fusion probe allows both fusion genes, generated by the t(4;11)(q21;q23) translocation, to be detected.
1. Meyer et al., Leukemia 2009;23(8):1490-9
2. Smith et al., Genes Dev. 2011;25(7): 661–72
3. Kumar et al., Leuk Res. 2011;35(3):305-9
4. Bursen et al., Blood. 2010; 29;115(17):3570-9
5. Professional Guidelines for Clinical Cytogenetics: ACUTE LYMPHOBLASTIC LEUKAEMIA BEST PRACTICEGUIDELINES (2011) V1.00. www.cytogenetics.org.uk
- Area of Interest*
This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples.
*Disease information supported by the literature and is not a reflection of the intended purpose of this product.